Translational profiling of hypothalamic and midbrain neurons that project to the nucleus accumbens.

ABSTRACT: Neuroanatomical methods enable high-resolution mapping of neural circuitry, but do not allow systematic molecular profiling of neurons based on their connectivity. Here, we report the development of a novel approach for molecularly profiling projective neurons. We show that ribosomes can be labeled with a camelid nanobody raised against GFP and that this system can be engineered to selectively capture translating mRNAs from cells expressing GFP. We generated a transgenic mouse encoding a nanobody-ribosomal protein fusion (Syn-NBL10) and used a retrograde virus (CAV) encoding GFP to immunoprecipitate ribosomes from projection neurons. This enabled us to profile neurons projecting to the nucleus accumbens. The current method provides a new means for profiling neurons based on their projectio

ORGANISM(S): Mus musculus

SUBMITTER: Jeffrey Friedman 

PROVIDER: E-GEOD-55800 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress