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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression of ultra violet irradiation of silk worm Bombyx mori larvae

ABSTRACT: 3 day of fifth instar larvae were treated with ultra-violet (UV) rays using UVL-56 (1350?W/cm2, UVP) for 6 and 12 hours (29.2 and 58.32 J/cm2). Since, larvae of each group rare on artificial diet for 24 hour and dissected fatbody. We examined gene expression profile in these groups. Gene expression was measured in 3 day of fifth instar larvae of ultra violet irradiation.

ORGANISM(S): Bombyx mori

SUBMITTER: Hiromasa Ono

PROVIDER: E-GEOD-55816 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Superoxide dismutases, SOD1 and SOD2, play a distinct role in the fat body during pupation in silkworm Bombyx mori.

Nojima Yosui Y Ito Katsuhiko K Ono Hiromasa H Nakazato Takeru T Bono Hidemasa H Yokoyama Takeshi T Sato Ryoichi R Suetsugu Yoshitaka Y Nakamura Yuki Y Yamamoto Kimiko K Satoh Jun-ichi J Tabunoki Hiroko H Fugo Hajime H

PloS one 20150225 2

One way that aerobic biological systems counteract the generation of reactive oxygen species (ROS) is with superoxide dismutase proteins SOD1 and SOD2 that metabolize superoxide radicals to molecular oxygen and hydrogen peroxide or scavenge oxygen radicals produced by the extensive oxidation-reduction and electron-transport reactions that occur in mitochondria. We characterized SOD1 and SOD2 of Bombyx mori isolated from the fat body of larvae. Immunological analysis demonstrated the presence of ...[more]

PMID: 25714339

Similar Datasets

Gene expression of ultra violet irradiation of silk worm Bombyx mori larvae

Project description:3 day of fifth instar larvae were treated with ultra-violet (UV) rays using UVL-56 (1350μW/cm2, UVP) for 6 and 12 hours (29.2 and 58.32 J/cm2). Since, larvae of each group rare on artificial diet for 24 hour and dissected fatbody. We examined gene expression profile in these groups.

2015-02-26 | GSE55816 | GEO

Gene expression of abnormal uric acid synthesis animal model of silk worm Bombyx mori.

Project description:Uric acid (UA) is the final product of purine metabolism and plays an important role as a physiological antioxidant. In recent years, several different groups have reported a correlation between decreased UA in Parkinson’s disease (PD) and clinical progression and stage of PD. However, little is known about the molecular mechanisms of decreased UA under oxidative stress. We used our systematic functional annotation pipeline for silkworm genes to identify a novel UA metabolic pathway regulator under oxidative stress in a UA metabolism mutant silkworm Bombyx mori model. Gene expression was measured in 3day of fifth instar larvae of abnormal uric acid synthesis Bombyx mori mutant of op.

2013-07-24 | E-GEOD-37735 | biostudies-arrayexpress

Protein atlas of four larval stages of Maruca vitrata

Project description:The present work is the pioneering report depicting the total proteomic atlas of four major developmental stages (third, fourth, fifth instar and pupa) of M. vitrata by LC-MS/MS analyses.

2023-11-20 | PXD043092 | Pride

Proteomic analysis of diapause and non-diapause stages of Loxostege sticticalis

Project description:Select five different treatment groups, including fifth instar larvae, pre diapause larvae, diapause larvae, diapause release treated larvae, and post diapause released larvae, for proteomic analysis

2023-12-21 | PXD048065 | iProX

Identification of lineage-specific cis-regulatory modules associated with variation in transcription factor binding and chromatin activity using Ornstein-Uhlenbeck models

Project description:Whole-chromatin profile (FAIRE-seq) in three Drosophila species (D. melanogaster, D. pseudoobscura and D. virilis) in eye-antennal imaginal discs at the stage of third instar wandering larvae. By the use of Ornstein-Uhlenbeck methods, we assess the evolutionary forces acting on regulatory elements (cis-level) on chromatin activity across Drosophila eye-antennal imaginal discs at the stage of third instar larvae.

2015-06-17 | E-GEOD-59706 | biostudies-arrayexpress

Functional opsin retrogene in nocturnal moth

Project description:RNA was isolated from first instar larvae, fifth instar larvae and adults using the TRIzol (Invitrogen) reagent by following the company manual. Poly(A) mRNA was isolated from 20 µl total RNA using Oligo (dT) magnetic beads and then was broken into short fragments (about 200bp) in the presence of fragmentation buffer at 94 °C for 5 min. The mRNA samples were used to construct the cDNA libray with mRNA-Seq assay, which was performed by Novogene (Beijing, China).The libraries were sequenced using HiSeq2000 or HiSeq4000 (Illumina) in paired-end mode, creating reads with a length of 101 bp (HiSeq2000) or 151 bp (HiSeq4000).

2017-02-22 | GSE86914 | GEO

Precocious metamorphosis of silkworm by BmNPV infection in the latter half of the fifth instar larvae

Project description:The silkworm, Bombyx mori, is a complete metamorphosis insect and an economically important for silk production, the model to study insect physiology and biochemistry. Bombyx mori nucleopolyhedrovirus (BmNPV) is a principal pathogen of the silkworm and its host range is restricted to silkworm larvae, requiring interaction with silkworm larvae to accomplish virus replication. Prothoracic glands (PGs) are a model for synthetic ecdysone with regulating insect growth and development. In this study, day-4 fifth instar silkworm larvae were infected by BmNPV, the wandering silkworms appeared in the infected groups were 12 hours earlier than that in the control groups, and the ecdysone titer in infected larvae was significantly higher than that of the control larvae. Then, we used RNA sequencing (RNA-seq) to analyze silkworm PGs 48 h after BmNPV infection. The classifications of the 15 differential expression genes (DEGs) were mainly involved in the metabolic processes and pathways. The RT-qPCR results of the DEGs in the PGs of BmNPV-infected at 24, 48, and 72 h were generally consistent with the transcriptome data. The transcripts of BmTrypsin-1 and BmACSS3 were significantly increased from 24 to 72 h after BmNPV infection that they may be involved in the maturation process in the latter half of silkworm fifth instar larvae. These findings will help to address the interactions between BmNPV infection and host developmental response.

2021-03-02 | GSE167875 | GEO

Bombyx mori

Project description:Gene expression of ultra violet irradiation of silk worm Bombyx mori larvae

| PRJNA241063 | ENA

Ibf contributes to CP190 recruitment to chromatin [ChIP-seq]

Project description:ChIP-Seq peak calling of CP190 in wild-type and Ibf2 mutant Drosophila melanogaster third instar larvae Two wild-type and two Ibf2 mutant Drosophila melanogaster third instar larvae were sequenced.

2014-04-18 | E-GEOD-52240 | biostudies-arrayexpress

GZ21T inhibits the growth of actinic keratoses in a UVB induced model of skin carcinogenesis

Project description:To investigate the mechanism behind GZ21T's ability to inhibit actinic keratoses growth in an ultra-violet B induced model of skin carcinogenisis

2023-02-02 | GSE223986 | GEO

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