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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of mouse neocortical and cerebellar astrocytes

ABSTRACT: Affymetrix Mouse Genome 430 2.0 GeneChip microarrays were used to analyze murine neocortical and cerbellar astrocytes generated from postnatal (PN) day 1 wild-type (ICR) pups. Experiment Overall Design: Three samples each of murine neocortical and cerebellar astrocytes were analyzed.

ORGANISM(S): Mus musculus

SUBMITTER: Mukesh Sharma

PROVIDER: E-GEOD-5582 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Distinct genetic signatures among pilocytic astrocytomas relate to their brain region origin.

Sharma Mukesh K MK Mansur David B DB Reifenberger Guido G Perry Arie A Leonard Jeffrey R JR Aldape Kenneth D KD Albin Meredith G MG Emnett Ryan J RJ Loeser Simon S Watson Mark A MA Nagarajan Rakesh R Gutmann David H DH

Cancer research 20070201 3

Pilocytic astrocytomas (PAs) are the most common glioma in children. Whereas many PAs are slow-growing or clinically indolent, others exhibit more aggressive features with tumor recurrence and death. To identify genetic signatures that might predict PA clinical behavior, we did gene expression profiling on 41 primary PAs arising sporadically and in patients with neurofibromatosis type 1 (NF1). Whereas no expression signature was found that could discriminate clinically aggressive or recurrent tu ...[more]

PMID: 17283119

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Neocortical and cerebellar astrocytes

Project description:Affymetrix Mouse Genome 430 2.0 GeneChip microarrays were used to analyze murine neocortical and cerbellar astrocytes generated from postnatal (PN) day 1 wild-type (ICR) pups. Keywords: neocortical astrocyte, cerebellar astrocyte, murine, postnatal day 1

2007-03-14 | GSE5582 | GEO

Layer-specific molecular expression in neocortical astrocytes

Project description:Layer-specific molecular expression in neocortical astrocytes

| PRJNA438534 | ENA

Comparative transcriptomic analysis of cerebellar astrocytes across developmental stages and brain regions

Project description:To profile gene expression differences in astrocytes in postnatal cerebellar astrocytes . Comparing cerebellar astrocyte transcriptomes at postnatal days 1, 7, 14, and 28 (P1, P7, P14, and P28),

2024-02-09 | GSE252519 | GEO

Mus musculus

Project description:Neocortical and cerebellar astrocytes

| PRJNA95447 | ENA

Layer-specific molecular expression in neocortical astrocytes

Project description:Protoplasmic astrocytes in layers II to VI of the mammalian neocortex have historically been thought to comprise a homogeneous population. Given that layer-specific neuronal subtypes play essential roles in cortical circuitry, astrocytes might also be expected to support and modify this circuitry in a layer-specific manner. In order to investigate whether protoplasmic astrocytes exhibit layer-specific heterogeneity, we compared the gene expression profiles of astrocytes between upper layers (layers II to IV) and deep layers (layers V and VI). Although most genes known to be preferentially expressed in astrocytes (astrocyte-enriched genes) were equally expressed between upper-layer astrocytes and deep-layer astrocytes, some such genes (astrocyte-enriched genes or genes with known function in astrocytes) were significantly enriched in upper-layer astrocytes or deep-layer astrocytes.

2018-04-06 | GSE111916 | GEO

Temporally defined neocortical translation and polysome assembly is determined by the RNA-binding protein, Hu antigen R

Project description:Precise spatiotemporal control of mRNA translation machinery is essential to proper development of highly complex systems like the neocortex. Here, we show that an RNA-binding protein, Hu antigen R (HuR), regulates both neocorticogenesis and specificity of neocortical translation machinery in a developmental stagedependent manner in mice. Neocortical absence of HuR alters the phosphorylation states of the initiation and elongation factors of the core translation machinery. In addition, HuR regulates the temporally specific positioning of functionally related mRNAs into the active translation sites, the polysomes. HuR also determines the specificity of neocortical polysomes by defining their combinatorial composition of ribosomal proteins and initiation and elongation factors. For some of the HuR-dependent proteins, the association with polysomes depends on the eIF2 alpha kinase 4 (eIF2ak4), which associated with HuR in prenatal developing neocortices. Finally, we found that deletion of HuR prior to embryonic day 10 (E10) disrupts both neocortical lamination and formation of the main neocortical commissure, the corpus callosum. Our study identifies a crucial role for HuR in neocortical development as a translational gatekeeper for functionally related mRNA subgroups and polysomal protein specificity. Cortex was dissected from mouse pups at embryonic day 13 (E13) or the day of birth (P0).

2014-08-25 | E-GEOD-50809 | biostudies-arrayexpress

Yin Yang 1 sets up the stage for cerebellar astrocyte maturation

Project description:Diverse subpopulations of astrocytes tile different brain regions to accommodate local requirements of neurons and associated neuronal circuits. Nevertheless, molecular mechanisms governing astrocyte diversity remain mostly unknown. We explored the role of a zinc finger transcription factor Yin Yang 1 (YY1) that is expressed in astrocytes. We found that specific deletion of YY1 from astrocytes causes severe motor deficits in mice, induces Bergmann gliosis, and results in simultaneous loss of GFAP expression in velate and fibrous cerebellar astrocytes. Single cell RNA-seq analysis showed that YY1 exerts specific effects on gene expression in subpopulations of cerebellar astrocytes. We found that although YY1 is dispensable for the initial stages of astrocyte development, it regulates subtype-specific gene expression during astrocyte maturation. Moreover, YY1 is continuously needed to maintain astrocyte identity in the adult cerebellum. Our findings suggest that YY1 plays critical roles regulating cerebellar astrocyte maturation during development and maintaining a mature phenotype of astrocytes in the adult cerebellum.

2022-04-01 | GSE166792 | GEO

Gene expression data from primary mouse neocortical cultures

Project description:Primary murine neocortical cultures are a common model for investigating fundamental attributes of neuronal signalling. Here we utilized an Affymetrix GeneChip platform to measure expression of various genes in vitro in order to chracterize our cortical cultures.

2022-11-23 | GSE218028 | GEO

BPTF is essential for murine neocortical development

Project description:BPTF is essential for murine neocortical development

| PRJNA647223 | ENA

Childhood cerebellar tumors mirror conserved fetal transcriptional programs

Project description:We used single-cell transcriptomics to study >60,000 cells from the developing murine cerebellum, and show that different molecular subgroups of childhood cerebellar tumors mirror the transcription of cells from distinct, temporally restricted cerebellar lineages.

| EGAS00001003170 | EGA

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