Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Whole genome gene expression profiling in CD4+ T-cells, FACS-sorted based on CADM1/CD7 expression levels, in Adult T-cell Leukemia-lymphoma (ATL) patients, HTLV-1 carriers, and in healthy volunteers.

ABSTRACT: CADM1 overexpresion and stepwise downregulation of CD7 in CD4+T-cells from ATL patients and HTLV-1 carriers accurately reflect the process of disease, i.e., oligoclonally and clonally exanding cells are accumulated in D (CADM1posCD7dim) and N (CADM1posCD7neg) populations, respectively. Moreover, gene expression profiling of each subpopulation supported that notion. PBMCs of asymptomatic carriers (ACs) and ATLs were subgrouped based on CADM1 and CD7 expression levels by multicolor flow cytometry. FACS-sorted subpopulations were then subjected to extraction of total RNA, followed by Cy-3 labeling and human whole genome gene expression microarray analyses.

ORGANISM(S): Homo sapiens

SUBMITTER: Kazumi Nakano 

PROVIDER: E-GEOD-55851 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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CADM1 expression and stepwise downregulation of CD7 are closely associated with clonal expansion of HTLV-I-infected cells in adult T-cell leukemia/lymphoma.

Kobayashi Seiichiro S   Nakano Kazumi K   Watanabe Eri E   Ishigaki Tomohiro T   Ohno Nobuhiro N   Yuji Koichiro K   Oyaizu Naoki N   Asanuma Satomi S   Yamagishi Makoto M   Yamochi Tadanori T   Watanabe Nobukazu N   Tojo Arinobu A   Watanabe Toshiki T   Uchimaru Kaoru K  

Clinical cancer research : an official journal of the American Association for Cancer Research 20140411 11

<h4>Purpose</h4>Cell adhesion molecule 1 (CADM1), initially identified as a tumor suppressor gene, has recently been reported to be ectopically expressed in primary adult T-cell leukemia-lymphoma (ATL) cells. We incorporated CADM1 into flow-cytometric analysis to reveal oncogenic mechanisms in human T-cell lymphotrophic virus type I (HTLV-I) infection by purifying cells from the intermediate stages of ATL development.<h4>Experimental design</h4>We isolated CADM1- and CD7-expressing peripheral bl  ...[more]

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