Detection of circRNA in Drosophila Fly heads and S2 cells using RNA-seq with or without RNaseR tretment

ABSTRACT: We determined circRNA abundance in fly Heads and S2 cells by generating and analyzing high-throughput RNA-sequencing libraries prepared from rRNA-depleted RNA. In order to determine whether the observed sequencing reads are due to bona fide circRNAs, we pre-treated the RNA with RNAse-R before the rRNA-depletion procedure. Indeed, most of the identified circRNAs were more enriched in comparison to the canonical mRNA isoforms following the RNAse-R treatment. We compare circRNA levels in wt (Canton S) flies with flies carrying the C4 ("slow polymerase") mutation. 4 samples of Drosophila Canton S and 4 samples of flies carrying the C4 ("slow polymerase") mutation. For each sample, one library was prepared from RNA after RNaseR treatment and the second from RNA with with

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Sebastian Kadener 

PROVIDER: E-GEOD-55872 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress