Dynamic study of In vitro murine follicle maturation in 3% alginate gels
Ontology highlight
ABSTRACT:
ORGANISM(S): Mus musculus
SUBMITTER: Beatriz Penalver Bernabe
PROVIDER: E-GEOD-55969 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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Project description:A microarray study to compare gene expression during ovarian follicle development to better understand transcriptional changes during follicular growth. Multi-layered secondary follicles (150-180 M-BM-5m in diameter) were mechanically isolated from ovaries of 16-day old CD-1 mice and individually encapsulated in 0.5% alginate. Follicles were culture for up to 8 days. Day 0 samples correspond to follicles that were isolated and immediately frozen. Samples were collected after 0, 2, 4, 5, 6 and 8 days in culture in triplicate and day 0 was collected 5 times.
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