Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and pro1 mutant Transcriptomes


ABSTRACT: Analysis of gene expression level. The hypothesis tested in the present study was that pro1 mutant influence the cell cycle pathway. Results provide important information of the cell cycle-related gene expression level regulation of cell cycle transition from G1 to S phase. Seedling mRNA profiles of 14DAG wild type (WT) and pro1 mutant were generated by deep sequencing, in triplicate, using Illumina Hiseq 2000 (Zea mays).

ORGANISM(S): Zea mays

SUBMITTER: Gang Wang 

PROVIDER: E-GEOD-56004 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Proline responding1 Plays a Critical Role in Regulating General Protein Synthesis and the Cell Cycle in Maize.

Wang Gang G   Zhang Jushan J   Wang Guifeng G   Fan Xiangyu X   Sun Xin X   Qin Hongli H   Xu Nan N   Zhong Mingyu M   Qiao Zhenyi Z   Tang Yuanping Y   Song Rentao R  

The Plant cell 20140620 6


Proline, an important amino acid, accumulates in many plant species. Besides its role in plant cell responses to environmental stresses, the potential biological functions of proline in growth and development are unclear. Here, we report cloning and functional characterization of the maize (Zea mays) classic mutant proline responding1 (pro1) gene. This gene encodes a Δ<sup>1</sup>-pyrroline-5- carboxylate synthetase that catalyzes the biosynthesis of proline from glutamic acid. Loss of function  ...[more]

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