KRAS and YAP1 converge to regulate EMT and tumor survival

ABSTRACT: Cancer cells that express oncogenic alleles of RAS typically require sustained expression of the mutant allele for survival, but the molecular basis of this oncogene dependency remains incompletely understood. To identify genes that can functionally substitute for oncogenic RAS, we systematically expressed 15,294 open reading frames in a human KRAS-dependent colon cancer cell line engineered to express an inducible KRAS-specific shRNA. We found 147 genes that promoted survival in the setting of KRAS suppression. In this model, the transcriptional co-activator YAP1 rescued cell viability in KRAS-dependent cells upon suppression of KRAS and was required for KRAS-induced cell transformation. Acquired resistance to Kras suppression in a Kras-driven murine lung cancer model also involved increa

ORGANISM(S): Mus musculus

SUBMITTER: A Bhutkar 

PROVIDER: E-GEOD-56175 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress