High-resolution analysis of gene activity during the Xenopus mid-blastula transition

ABSTRACT: The Xenopus mid-blastula transition (MBT) marks the onset of large-scale zygotic transcription, as well as an increase in cell cycle length and a loss of synchronous cell divisions. Little is known about what triggers the activation of transcription or how newly expressed genes interact with each other. Here we use high-resolution expression profiling to identify three waves of gene activity: a post-fertilization wave involving polyadenylation of maternal transcripts; a broad wave of zygotic transcription detectable as early as the 7th cleavage and extending beyond the MBT at the 12th cleavage; and a shorter post-MBT wave of transcription that becomes apparent as development proceeds. Our studies have also allowed us to define a set of maternal genes whose mRNAs are deadenylated shortly af

ORGANISM(S): Xenopus (Silurana) tropicalis

SUBMITTER: Mike Gilchrist 

PROVIDER: E-GEOD-56242 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress