Lenti-miR Library SW620, Pre vs Post Intrahepatic Selection

ABSTRACT: Cells were transduced with a lentiviral Lenti-miR library composed of 661 miRNAs (System Biosciences) at a low multiplicity of infection (MOI) such that each cell over-expressed a single miRNA. The transduced population was then injected intra-hepatically into NOD-SCID mice for in vivo selection of miRNAs that when over-expressed, either promoted or suppressed metastatic liver colonization. Genomic DNA PCR amplication and recovery of lenti-viral miRNA inserts was performed on cells prior to injection, and from liver nodules, according to the manufacturer’s protocol. miRNA array profiling allowed for miRNA insert quantification prior to and subsequent to in vivo selection. To use an unbiased approach to identify regulators of colon cancer metastasis, we transduced a population of colon canc

ORGANISM(S): Homo sapiens

SUBMITTER: Jia Min Loo 

PROVIDER: E-GEOD-56318 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress