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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Next generation sequencing of Phaeodactylum tricornutum grown with and without nitrogen

ABSTRACT: We have done next generation sequencing of optically thin, exponentialy growing Phaeodactylum tricornutum cultures grown with and without nitrogen source to improve our undestanding of the pathways regulation under conditions that promote lipid accumulation (N-starvation). 3 biologically independent exponentially growing culture of Phaeodactylum tricornutum were pelleted and washed several times with N-free media. Each culture was devided to 2 replicates with initial cell concentration of 2X105 cells/mL, one with NaNO3 as nitrogen source and the other without any nitrogen source. The cuture were bubbled foro 48 hours and sampled for transcriptome together with other physiological parameters and lipid analysis.

ORGANISM(S): Phaeodactylum tricornutum

SUBMITTER: Orly Levitan

PROVIDER: E-GEOD-56346 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Remodeling of intermediate metabolism in the diatom Phaeodactylum tricornutum under nitrogen stress.

Levitan Orly O Dinamarca Jorge J Zelzion Ehud E Lun Desmond S DS Guerra L Tiago LT Kim Min Kyung MK Kim Joomi J Van Mooy Benjamin A S BA Bhattacharya Debashish D Falkowski Paul G PG

Proceedings of the National Academy of Sciences of the United States of America 20141229 2

Diatoms are unicellular algae that accumulate significant amounts of triacylglycerols as storage lipids when their growth is limited by nutrients. Using biochemical, physiological, bioinformatics, and reverse genetic approaches, we analyzed how the flux of carbon into lipids is influenced by nitrogen stress in a model diatom, Phaeodactylum tricornutum. Our results reveal that the accumulation of lipids is a consequence of remodeling of intermediate metabolism, especially reactions in the tricarb ...[more]

PMID: 25548193

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Next generation sequencing for understading the role of nitrate reductase in remodeling intermediate metabolism in Phaeodactylum tricornutum

Project description:We have done next generation sequencing of optically thin, exponentialy growing WT Phaeodactylum tricornutum and our ~50% nitrate reductase knock-down cultures. Culture were grown with and without nitrogen source to improve our undestanding of the pathways regulation under conditions that promote lipid accumulation (N-starvation). 3 biologically independent exponentially growing culture of the WT and the NR21 knock-down were pelleted and washed several times with N-free media. Each culture was devided to 2 replicates with initial cell concentration of 2.5X105 cells/mL, one with NaNO3 as nitrogen source and the other without any nitrogen source. The cuture were bubbled for 48 hours and sampled for transcriptome together with other physiological parameters and lipid analysis after reachign exponential growth.

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