Gene expression analysis of FOXN1+ thymic endoderm cells derived from the in vitro differentiation of human embryonic stem cells

ABSTRACT: The goal of this study was to document the gene expression profile of FOXN1+ thymic endoderm cells derived from the in vitro differentiation of human pluripotent stem cells. Thymic epithelial cells (TECs) play a critical role in T-cell maturation and tolerance induction. The generation of TECs from in vitro differentiation of human pluripotent stem cells (PSCs) would provide a platform for studying the mechanisms of this interaction and have implications for immune reconstitution. To facilitate analysis of PSC-derived TECs, we generated human embryonic stem cell (hESC) reporter lines in which sequences encoding GFP were targeted to FOXN1, a gene required for TEC development. Using this FOXN1GFP/w line as a read out, we developed a reproducible protocol for generating FOXN1-GFP+ thymic endo

ORGANISM(S): Homo sapiens

SUBMITTER: Ed Stanley 

PROVIDER: E-GEOD-56373 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress