DNA methylation in adult queend and worker bees

ABSTRACT: In honey bees (Apis mellifera) the behaviorally and reproductively distinct queen and worker female castes derive from the same genome as a result of differential intake of royal jelly and are implemented in concert with DNA methylation. To determine if these very different diet-controlled phenotypes correlate with unique brain methylomes, we conducted a study to determine the methyl cytosine (mC) distribution in the brains of queens and workers at single-base-pair resolution using shotgun bisulfite sequencing technology. The whole-genome sequencing was validated by deep 454 sequencing of selected amplicons representing eight methylated genes. We found that nearly all mCs are located in CpG dinucleotides in the exons of 5,854 genes showing greater sequence conservation than non-methylated

ORGANISM(S): Apis mellifera

SUBMITTER: Sylvain Foret 

PROVIDER: E-GEOD-56399 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress