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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Induced expression of the C19MC miRNAs in HTR-8/SVneo cells (microRNA)

ABSTRACT: Analyze miRNA expression levels in HTR-8/SVeo cells stably transfected with a BAC plasmid containing the entire C19MC miRNA cluster HTR-8/SVeo cells were transfected with a modified BAC plasmid containing the entire C19MC miRNA cluster and carrying a zeocin selection cassette. Several independent clones and a mixed population of transfected cells were analyzed and compared to non-transfected HTR-8/SVeo cells and primary human trophoblasts that express the C19MC miRNAs endogenously

ORGANISM(S): Homo sapiens

SUBMITTER: Jean-Francois Mouillet

PROVIDER: E-GEOD-56562 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Induced expression of the C19MC miRNAs in HTR-8/SVneo cells (mRNA)

Project description:Analyze gene expression profiles in HTR-8/SVeo cells stably transfected with a BAC plasmid containing the entire C19MC miRNA cluster HTR-8/SVeo cells were transfected with a modified BAC plasmid containing the entire C19MC miRNA cluster and carrying a zeocin selection cassette. Several independent clones and a mixed population of transfected cells were analyzed and compared to non-transfected HTR-8/SVeo cells and primary human trophoblasts that express the C19MC miRNAs endogenously

2014-04-08 | E-GEOD-56564 | biostudies-arrayexpress

Induced expression of the C19MC miRNAs in HTR-8/SVneo cells (microRNA)

Project description:Analyze miRNA expression levels in HTR-8/SVeo cells stably transfected with a BAC plasmid containing the entire C19MC miRNA cluster

2014-04-08 | GSE56562 | GEO

Induced expression of the C19MC miRNAs in HTR-8/SVneo cells (mRNA)

Project description:Analyze gene expression profiles in HTR-8/SVeo cells stably transfected with a BAC plasmid containing the entire C19MC miRNA cluster

2014-04-08 | GSE56564 | GEO

Induced expression of the C19MC miRNAs in HTR-8/SVneo cells

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2014-04-08 | E-GEOD-56565 | biostudies-arrayexpress

Genome-wide analysis of SPARC(secreted protein acidic and rich in cysteine)-responsive gene expression in HTR-8/SVneo cells

Project description:SPARC is a matricellular glycoprotein that plays critical roles in the pathologies associated with obesity and diabetes, as well as tumorigenesis. The objective of this study was to investigate the role of SPARC in the process of trophoblast invasion which shares many similarities with tumor cells invasion. Our results reveals that hormones, cell adhesion molecules, ECM molecules, growth factors and cytokines all are mediated by SPARC in EVT invasion. HTR-8/SVneo cells were transfected with SPARC siRNA or a 25-nucleotide universal negative control siRNA using Lipofectamine 2000 according to the manufacturer’s protocol. Seventy-two hours after transfection, cells were harvested and RNA was isolated using standard procedures.

2012-04-27 | E-GEOD-37639 | biostudies-arrayexpress

Effect of IL-11 on gene expression in JEG-3 and HTR-8/SVneo trophoblastic cells

Project description:IL-11 increases the invasiveness of JEG-3 cells while, reduces the invasiveness of HTR-8/SVneo cells. This study examines the effect of IL-11 on gene expression in trophobalstic cell models viz. JEG-3 and HTR-8/SVneo cells to resolve the controversies associated with the IL-11 mediated regulation of the invasiveness of these two cell lines. JEG-3 and HTR-8/Svneo cells were stimulated with IL-11 (200 ng/ml) for 24 h in pain medium keeping un-treated cells as control. After 24 h of stimulation, total RNA was isolated from these cells and used for the microarray experiments. These experiments were performed once.

2011-11-04 | E-GEOD-31608 | biostudies-arrayexpress

Effect of LIF and IL-6 on gene expression in JEG-3 and HTR-8/SVneo trophoblastic cells

Project description:Both leukemia inhibitory factor (LIF) and interleukin-6 (IL-6) increase the invasiveness of JEG-3 and HTR-8/SVneo cells. This study examines the effect of LIF and IL-6 on gene expression in trophoblastic cell models viz. JEG-3 and HTR-8/SVneo cells to decipher the molecular basis of the increase in invasiveness. JEG-3 and HTR-8/Svneo cells were stimulated with LIF (50 ng/ml) and IL-6 (100 ng/ml) for 24 h in plain medium keeping untreated cells as a control. After 24 h of stimulation, total RNA was isolated from these cells and used for microarray experiments. These experiments were performed once.

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2010-06-22 | E-GEOD-17501 | biostudies-arrayexpress

The Assembly of miRNA-mRNA-protein Regulatory Networks Using High-throughput Expression Data (miRNA)

Project description:Analyze miRNA expression levels in primary trophoblasts, derived from term human placenta and cultured under standard or hypoxic conditions Human placental trophoblasts were dispersed using a trypsin-deoxyribonuclease-dispase/Percoll method, plated in 6-well plates, and maintained in standard culture conditions (O2=20%). After 4 h (defined as time 0), the plates were divided to those in continued standard culture conditions, or to culture in hypoxia (O2=0%). Cells were then harvested at 6 h, 12 h, 24 h, 48 h and 72 h, and processed for miRNA arrays

2015-03-01 | E-GEOD-60431 | biostudies-arrayexpress

Expression profiles of X-ray responsive miRNA in human cells

Project description:MicroRNA (miRNA) is a type of non-coding RNA that regulates the expression of its target genes by interacting with the complementary sequence of the target mRNA molecules. Recent evidence has shown that genotoxic stress induces miRNA expression, but the target genes involved and role in cellular responses remain unclear. We examined the role of miRNA in the cellular response to X-ray irradiation by studying the expression profiles of radio-responsive miRNAs and their target genes in cultured human cell lines. We found that expression of miR-574-3p was induced in the lung cancer cell line A549 by X-ray irradiation. Overexpression of miR-574-3p caused delayed growth in A549 cells. A predicted target site was detected in the 3'-untranslated region of the enhancer of the rudimentary homolog (ERH) gene, and transfected cells showed an interaction between the luciferase reporter containing the target sequences and miR-574-3p. Overexpression of miR-574-3p suppressed ERH protein production and delayed cell growth. This delay was confirmed by knockdown of ERH expression. Our study suggests that miR-574-3p may contribute to the regulation of the cell cycle in response to X-ray irradiation via suppression of ERH protein production. This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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