RNA-Sequencing Identifies Novel Imprinted Genes in Human Placenta (SNP genotyping)

ABSTRACT: Fetal health is dependent upon the epigenetic-based regulation of gene expression in placenta. Genomic imprinting is an epigenetic phenomenon common to placenta and refers to the monoallelic expression of a gene in a parental-specific manner. We aimed to detect novel imprinted genes in human placenta by applying whole transcriptome RNA-sequencing and genotyping of coding variants. Ten family trios with healthy spontaneous single term pregnancy were recruited. Parental and child DNA genotypes were analysed using exome SNP genotyping microarrays, revealing the inheritance of parental alleles. Total RNA was extracted from placental tissue for whole transcriptome analysis. The imprinted genes showed consistent expression from either parental allele as demonstrated by the SNP content of sequenc

ORGANISM(S): Homo sapiens

SUBMITTER: Tauno Metsalu 

PROVIDER: E-GEOD-56685 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress