Effects of knockdown of ROCK1 and ZIPK on gene expression in cultured human vascular smooth muscle cells

ABSTRACT: Rho-associated kinase (ROCK) and zipper-interacting protein kinase (ZIPK) have been implicated in diverse physiological functions, including smooth muscle contraction, cell proliferation, cell adhesion, apoptosis, cell migration and inflammation. Many aspects of regulation via ROCK and ZIPK, however, remain unclear. In this study, we utilized an siRNA approach to knock down ROCK1 and ZIPK in cultured human arterial smooth muscle cells. Microarray analysis was performed, using a whole-transcript expression chip, to identify changes in gene expression profiles induced by ROCK1 and ZIPK knockdown. ROCK1 knockdown affected the expression of 553 genes (355 down-regulated and 198 up-regulated), while ZIPK knockdown affected the expression of 390 genes (219 down-regulated and 171 up-regulated). A

ORGANISM(S): Homo sapiens

SUBMITTER: Michael Walsh 

PROVIDER: E-GEOD-56819 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress