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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Regulation of gene expression by JMJD5 in LNCaP prostate cancer cells

ABSTRACT: The goal of this study was to define the cohort of genes whose expression is regulated by JMJD5 function in prostate cancer. Towards this goal, LNCaP sublines stably expressing wld-type JMJD5 (LNCaP-JMJD5) were established. In addition, a control subline(LNCaP-Vector) was generated by transfection with empty vector. Both sublines were cultured in either androgen-deprived medium or in the presence of dihydrotestosterone (DHT). Subsequently, total RNA was isolated and then subjected to microarray gene expression profiling with Affymetrix GeneChip HG-U133 Plus 2.0 Arrays. A total of 4 samples were analyzed in this study. The study included two cell lines, LNCaP-Vector and LNCaP-JMJD5, that were cultured under conditions of androgen depivation (-DHT) or supplementation with dihydrotestosterone (+DHT). The LNCaP-Vector cell line served as the control for the study.

ORGANISM(S): Homo sapiens

SUBMITTER: Clifford Tepper

PROVIDER: E-GEOD-56908 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Ligand-Dependent Enhancer Activation Regulated by Topoisomerase-I Activity

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Regulation of gene expression by JMJD5 in LNCaP prostate cancer cells

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2013-04-01 | E-GEOD-44905 | biostudies-arrayexpress

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2013-02-05 | E-GEOD-39879 | biostudies-arrayexpress

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2011-01-07 | E-GEOD-26483 | biostudies-arrayexpress

LNCaP cells in response to DHT stimulation and inhibition/knockdown of TOPO2B

Project description:Microarray experiments were carried out to ascertain whether TOP2Î² is required for DHT induced androgen receptor target gene expression. We investigated the effect of pharmacological inhibition or RNA interference-mediated depletion of TOP2Î² on gene expression in androgen-dependent LNCaP prostate cancer cells. Analysis of gene expression in LNCaP cells under various conditions including serum starvation, DHT treatment, and DHT treatment combined with TOPO2B pharmacological inhibitors (Merbarone and Etoposide) and TOPO2B-shRNA knockdown.

2010-07-29 | E-GEOD-19445 | biostudies-arrayexpress

Transcription profiling of human LNCaP cells to investigate androgen receptor mediated gene expression by a sequence-specific DNA binding polyamide

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