Generation of CNS neural stem cells and PNS derivatives from neural crest derived peripheral stem cells [Dataset 1]

ABSTRACT: Neural crest-derived neural stem cells (NCSCs) from the embryonic PNS can be reprogrammed in neurosphere culture (NS) to rNCSCs that produce CNS progeny, including myelinating oligodendrocytes. Using global gene expression analysis we now demonstrate that rNCSCs completely lose their previous PNS characteristics and acquire the identity of neural stem cells derived from embryonic spinal cord (SCSCs). Reprogramming proceeds rapidly and results in a homogenous population of Olig2-, Sox3- and Lex-positive CNS stem cells. Low-level expression of pluripotency inducing genes Oct4, Nanog and Klf4 argues against a transient pluripotent state during reprogramming. The acquisition of CNS properties is prevented in the presence of BMP4 (BMP NCSCs) as shown by marker gene expression and the potential

ORGANISM(S): Mus musculus

SUBMITTER: Hermann Rohrer 

PROVIDER: E-GEOD-56999 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress