Controlling for gene expression changes in transcription factor protein networks.

ABSTRACT: The development of affinity purification technologies together with mass spectrometric analyses of the purified protein mixtures (AP-MS) has been used both to identify new protein-protein interactions and to define the subunit composition of protein complexes. Transcription factor protein interactions, however, have not been systematically analyzed using these approaches. Here, we have investigated whether ectopic expression of an affinity tagged transcription factor as bait in AP-MS experiments perturbs gene expression in cells resulting in false positive identification of bait associated proteins when typical experimental controls are used. Using quantitative proteomics and RNA-Seq, we determined that the increase in the abundance of a set of proteins caused by overexpression of the tran

ORGANISM(S): Homo sapiens

SUBMITTER: Chris Seidel 

PROVIDER: E-GEOD-57027 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress