Localization and Abundance Analysis of Human lncRNAs at Single Cell and Single Molecule Resolution

ABSTRACT: Long noncoding RNAs (lncRNAs) have emerged as key players in different cellular processes and are required for diverse functions in vivo. However, fundamental aspects of lncRNA biology remain poorly characterized, including their subcellular localization, abundance and variation at a single cell resolution. Here, we used single molecule, single-cell RNA fluorescence in situ hybridization (RNA FISH) to survey 61 lncRNAs, chosen by properties such as conservation, tissue specific expression, and expression abundance, and to catalog their abundance and cellular localization patterns in three human cell types. Our lncRNAs displayed diverse sub-cellular localization patterns ranging from strictly nuclear localization to almost exclusive cytoplasmic localization, with the majority localized prim

ORGANISM(S): Homo sapiens

SUBMITTER: Nataly Cabili 

PROVIDER: E-GEOD-57049 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress