Expression analysis of an enrofloxacin and a tetracline resistant Escherichia coli strain

ABSTRACT: Comparison of the whole genome gene expression level of an enrofloxacin and tetracycline resistant E. coli strain with the wildtype it was derived from. The process of drug adaptation of E. coli MG1655 wildtype cells is further descibed in van der Horst, M, J.M. Schuurmans, M. C. Smid, B. B. Koenders, and B. H. ter Kuile (2011) in Microb. Drug Resist. 17:141-147. Resistance to amoxicillin was induced in E. coli by growth in the presence of stepwise increasing antibiotic concentrations. To investigate consequences of the aquisition of amoxicillin resistance the transcriptomic profile of sensitive and resistant cells was compared in the absence and presence of sub-inhibitory (0.25xMIC) amoxicillin concentrations was compared. Total RNA of 3 biological replicates of E. coli MG1655 wildtype ce

ORGANISM(S): Escherichia coli str. K-12 substr. MG1655

SUBMITTER: Nadine HM-CM-$ndel 

PROVIDER: E-GEOD-57084 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress