Analysis of the Epigenomic and Transcriptional Landscapes during mammalian spermatogenesis [sperm; ChIP-seq]

ABSTRACT: Adult germline stem cells (AGSCs) are multifunctional - they must self renew, maintain genome pluripotency, and prepare for gametogenesis – which involves meiotic and chromatin repackaging phases. To better understand AGSCs and gametogenesis, we derived high-resolution profiles of transcription, DNA methylation, 5hmC, and multiple histone modifications at key stages. First, AGSCs display chromatin ‘poising’ of enhancers and promoters of genes utilized in embryo development. Second, the pluripotency network in AGSCs is remarkably distinct from ESCs - lacking Nanog, Sox2, or Prdm14 expression.  Third, spermatogenesis involves stage-specific transcription and distinctive chromatin dynamics, but virtually no changes in DNAme.  Surprisingly, we observe co-incidence of RNA polymerase II, high H3

ORGANISM(S): Homo sapiens

SUBMITTER: Diana Low 

PROVIDER: E-GEOD-57095 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress