Assessment of the Osteoblast Transcriptome in a Model of Markedly Enhanced Intramembranous Bone Formation Due to Constitutive Gs-G Protein Signaling in Osteoblasts

ABSTRACT: G protein coupled receptor (GPCR) signaling in osteoblasts (OBs) is an important regulator of bone formation. We previously described a mouse model expressing Rs1, an engineered constitutively active Gs-coupled GPCR, under the control of the 2.3 kb-Col I promoter. These mice showed a dramatic age-dependent increase in trabecular bone which were accompanied by an increase in OB lineage cells, especially immature OBs, indicated by an expansion of cells expressing Osterix and Runx2 in the whole femur. In this study, we further evaluated how Gs signaling in OBs affects intramembranous bone formation by examining calvariae of one-and nine-week-old Col1(2.3)/Rs1 mice. Rs1 calvariae displayed a dramatic increase in total volume and trabecular bone volume with partial loss of cortical structure. B

ORGANISM(S): Mus musculus

SUBMITTER: Lalita Wattanachanya 

PROVIDER: E-GEOD-57104 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress