Expression data from Spade/Spade mutant and wild type littermates whole ear tissue

ABSTRACT: Spade/Spade mutant develop the spontaneous progressive atopic dermatitis like skin inflammation at around 8-10 weeks after birth. In order to detect the pre-symptomatic events that occur in their ear tissue, we have sampled RNA from the whole ear tissue and identified the differentially expressed genes with twofolds difference in comparison with littermate wildtype ear tissue RNA samples. RNA from ear tissue was isolated using an RNeasy Fibrous Tissue Kit (Qiagen) and microarray analysis was performed using GeneChip mouse genome 430_2.0 array (Affymetrix). The data were analyzed by GeneSpring GX (Agilent Technologies). The data were further analyzed by using packages in R and Bioconductor(48). Per chip normalization of datasets was performed by GCRMA package. An average intensity of two

ORGANISM(S): Mus musculus

SUBMITTER: Hisahiro Yoshida 

PROVIDER: E-GEOD-57108 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress