Illumina Sequencing data of Insulator Proteins and the co-factor dMes-4

ABSTRACT: The Drosophila insulator-binding proteins (IBPs) dCTCF/Beaf32 mark the physical borders of chromosomal domains involving co-factors that participate in long-range interactions. Chromosomal borders are further enriched in specific histone modifications yet the implication of histone modifiers and nucleosome dynamics remains largely unknown in such context. Here, we show that IBP depletion impairs nucleosome dynamics over genes flanked by their binding sites. Biochemical purification identifies a key histone methyltransferase of H3K36, NSD/dMes-4, as a novel co-factor of IBPs involved in chromatin accessibility, which specifically co-regulates hundreds of genes flanked by Beaf32/dCTCF. dMes-4 presets chromatin before the recruitment of transcriptional activators including DREF that triggers

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Olivier Cuvier 

PROVIDER: E-GEOD-57165 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress