An in vivo screen identifies compounds that disrupt heterochromatin integrity

ABSTRACT: Heterochromatin plays a key role in gene repression, maintaining genome integrity and chromosome segregation. Fission yeast, Schizosaccharomyces pombe, utilizes conserved components to direct heterochromatin formation using siRNA generated by RNA interference (RNAi) to guide a histone H3 lysine 9 methyltransferase to cognate chromatin. To identify compounds that inhibit heterochromatin formation, an in vivo phenotypic screen for loss of silencing was performed. A tester strain harbouring a silent dominant selectable kanMX reporter gene within fission yeast centromeric heterochromatin was used to screen a diverse library of chemicals. HMS-I1 and HMS-I2 were identified as compounds that reproducibly increased G418 resistance due to loss of kanMX silencing, and decreased the level of repress

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Karl Ekwall 

PROVIDER: E-GEOD-57207 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress