A global regulatory mechanism for activating an exon network required for neurogenesis

ABSTRACT: The vertebrate and neural-specific SR-related protein nSR100/SRRM4 regulates an extensive program of alternative splicing with critical roles in nervous system development. However, the mechanism by which nSR100 controls its target exons is poorly understood. We demonstrate that nSR100-dependent neural exons are associated with a unique configuration of intronic cis-elements that promote rapid switch-like regulation during neurogenesis. A key feature of this configuration is the insertion of specialized intronic enhancers between polypyrimidine tracts and acceptor sites that bind nSR100 to potently activate exon inclusion in neural cells, while weakening 3' splice site recognition and contributing to exon skipping in non-neural cells. nSR100 further operates by forming multiple interaction

ORGANISM(S): Mus musculus

SUBMITTER: Bushra Raj 

PROVIDER: E-GEOD-57278 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress