Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Culex pipiens pallens and Cx. p. quinquefasciatus with different resistant level

ABSTRACT: Culex pipiens pallens and Cx. p. quinquefasciatus are important vectors of many diseases, such as West Nile fever and lymphatic filariasis. The widespread use of insecticides to control these disease vectors and other insect pests has led to insecticide resistance becoming common in these species. High throughput screening using SSH and specific microarray platforms was thought to have identified some resistance-related genes. However, limitations of these methods meant that only a few hundred of the many thousand genes could be screened. It wasn’t until the sequencing of the Cx. quinquefasciatus genome in 2010 that it became possible to screen all 18.9 thousand genes in the mosquito genome for anti-insecticidal activity. We used high throughout Illumina sequencing to identify hundreds of Cx. p. pallens and Cx. p. quinquefasciatus genes that were differentially expressed in response to pesticide exposure. The identification of these genes is a vital first step for more detailed investigation of the molecular mechanisms involved in insecticide resistance in mosquitoes. In this study, larvae of Cx. pipiens pallens and Cx. pipiens quinquefasciatus were collected from field and transported to the laboratory and reared to adulthood to get F1 generation. Then, half of the F1 generation was conducted to pesticide bioassay. RNA extraction and Illumina sequencing were undertaken in another half of the F1 generation. Therefore, Samples used in Illumina sequencing did not contact any insecticides. Twelve Cx. pipiens pallens and Cx. pipiens quinquefasciatus lavae were undertaken Illumina RNA sequencing.

ORGANISM(S): Culex pipiens pallens

SUBMITTER: Chunxiao Li

PROVIDER: E-GEOD-57307 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:The heavy use of pesticides in intensive agricultural areas often leads to the contamination of neighbouring mosquito larvae breeding sites. Such exposure to complex agrochemical mixtures can affect the tolerance of mosquitoes larvae to insecticides. The objective of this study was to determine whether agrochemical pollutants found in Anopheline larval breeding sites can affect the tolerance of adults to Fludora® Fusion, a novel vector control insecticide formulation combining two insecticides with distinct modes of action. An. gambiae larvae were exposed to a sub-lethal dose of a mixture of agrochemical pesticides representative of a region with an intense agricultural activity in Ivory Coast. Comparative bioassays with Fludora® Fusion and its two insecticide component (deltamethrine and clothianidin) were carried out between adult mosquitoes exposed or not to the agrochemicals at the larval stage. A transcriptomic analysis through RNA sequencing was subsequently performed in larvae and adults in order to highlight the molecular mechanisms underlying the phenotypic changes observed. Bioessais revealed a significant increased tolerance of adult females to clothianidin (2.5-fold) and Fludora® Fusion (2.2-fold) following larval exposure to agrochemicals. No significant increased tolerance to deltamethrin was observed suggesting that pesticide exposure affects the efficacy of the Fludora® Fusion mixture mainly through mechanisms acting on clothianidin tolerance. Transcriptomic analysis revealed the potential of agrochemicals to induce various resistance mechanisms including cuticle proteins, detoxifications activities and altered insecticide sequestration. These results suggest that though Fludora® Fusion has a good potential for vector control, its efficacy may be locally affected by the ecological context. The present study also suggests that, although the complex interactions between the use of agrochemicals and vector control insecticides are difficult to decipher in the field, they still must be considered in the frame of insecticide resistance management programmes.

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Culex pipiens pallens and Cx. p. quinquefasciatus with different resistant level

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