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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from Suv420h1 knockout and mutant PREX2 expressing MEFs (mouse embryonic fibroblasts)

ABSTRACT: PREX2 truncating mutations occur in melanoma. We used microarray based gene expression profiling to compare expression patterns between cells harboring Suv420h1 knockout and PREX2 mutant expressing Spontaneously immortalized MEFs from either wt or Suv420h1 knockout mice were used. Wt MEFs were transduced with lentivirus to express indicated PREX2 mutants. Suv420h1 ko MEFs were reconstituted either with control GFP or Suv420h1-GFP fusion. Total RNA was isolated and subjected to analysis

ORGANISM(S): Mus musculus

SUBMITTER: Lynda Chin

PROVIDER: E-GEOD-57310 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Truncating PREX2 mutations activate its GEF activity and alter gene expression regulation in NRAS-mutant melanoma.

Lissanu Deribe Yonathan Y Shi Yanxia Y Rai Kunal K Nezi Luigi L Amin Samir B SB Wu Chia-Chin CC Akdemir Kadir C KC Mahdavi Mozhdeh M Peng Qian Q Chang Qing Edward QE Hornigold Kirsti K Arold Stefan T ST Welch Heidi C E HC Garraway Levi A LA Chin Lynda L

Proceedings of the National Academy of Sciences of the United States of America 20160216 9

PREX2 (phosphatidylinositol-3,4,5-triphosphate-dependent Rac-exchange factor 2) is a PTEN (phosphatase and tensin homolog deleted on chromosome 10) binding protein that is significantly mutated in cutaneous melanoma and pancreatic ductal adenocarcinoma. Here, genetic and biochemical analyses were conducted to elucidate the nature and mechanistic basis of PREX2 mutation in melanoma development. By generating an inducible transgenic mouse model we showed an oncogenic role for a truncating PREX2 mu ...[more]

PMID: 26884185

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