Galectin-3 Deficiency Exacerbates Hyperglycemia and the Endothelial Response to Diabetes

ABSTRACT: Microarray analyses were performed in order to determine the effect of galectin-3 ablation on the endothelial transcriptional response in a mouse model of type 2 diabetes. Galectin-3-deficient mice (KO) and wild-type C57BL/6 (WT) were fed a high-fat diet (60% fat calories) or standard chow for 8 weeks. CD105+/CD45- endothelial cells were isolated from the aortae and skeletal muscles of these mice by FACS. Whole genome microarray expression profiling revealed greater transcriptional dysregulation in the endothelium of the KO after high-fat feeding compared to WT. Transcripts dysregulated in the KO endothelium after HFD include those involved in glucose uptake and insulin signaling, oxidative stress, vasoregulation, coagulation, and atherogenesis. Real-time PCR confirmed transcriptional down

ORGANISM(S): Mus musculus

SUBMITTER: April Darrow 

PROVIDER: E-GEOD-57329 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress