Stage-specific regulation of translation revealed by ribosome profiling of Trypanosoma brucei

ABSTRACT: Trypanosoma brucei subspecies infect humans and animals in sub-Saharan Africa. This early diverging eukaryote shows many novel features in basic biological processes, including the use of polycistronic transcription to generate all protein-coding mRNAs. Therefore we hypothesized that translational control provides a means to tune gene expression during parasite development in mammalian and fly hosts. We used ribosome profiling to examine genome-wide protein production in animal-derived slender bloodstream forms and cultured procyclic (insect midgut) forms. About one-third of all CDSs showed statistically significant regulation of protein production between the two stages. Of these, more than two-thirds showed a change in translation efficiency, but few were controlled by this parameter

ORGANISM(S): Trypanosoma brucei

SUBMITTER: Gowthaman Ramasamy 

PROVIDER: E-GEOD-57336 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress