The Global Landscape of Transcription Initiation During the Caulobacter Cell Cycle [mapping]

ABSTRACT: The Caulobacter cell cycle includes in an asymmetric cell division that is driven by a core regulatory circuit comprised of 4 transcription factors (DnaA, GcrA, CtrA, and SciP) and a DNA methyltransferase (CcrM). Using a modified global 5M-bM-^@M-^Y RACE protocol we mapped 2,726 transcriptional start sites (TSS) in the 4mb Caulobacter genome and identified 586 cell cycle-regulated TSS. The core cell cycle circuit directly controls about 55% of cell cycle-regulated TSS by integrating multiple regulatory inputs within at least 322 promoters, providing a large number of transcription profiles from a small number of regulatory factors. Here, we identified previously unknown features of the core cell cycle circuit, including antisense TSS within dnaA and ctrA, plus newly identified TSS for

ORGANISM(S): Caulobacter crescentus NA1000

SUBMITTER: Jared Schrader 

PROVIDER: E-GEOD-57365 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress