Gene expression profiling of human peripheral blood lymphocytes cultured in modeled microgravity

ABSTRACT: In the present study we analyzed miRNA and mRNA expression profiles in human peripheral blood lymphocytes (PBLs) incubated in microgravity condition, simulated by a ground-based Rotating Wall Vessel (RWV) bioreactor. Our results show that 42 miRNAs were differentially expressed in MMG-incubated PBLs compared with 1g-incubated ones. Among these, miR-9-5p, miR-9-3p, miR-155-5p, miR-150-3p, and miR-378-3p were the most dysregulated. To improve the detection of functional miRNA-mRNA pairs we performed gene expression profiles on the same samples assayed for miRNA profiling and we integrated miRNA and mRNA expression data. The functional classification of miRNA-correlated genes evidenced significant enrichments in the biological processes of immune/inflammatory response, signal transduction, re

ORGANISM(S): Homo sapiens

SUBMITTER: Gerolamo Lanfranchi 

PROVIDER: E-GEOD-57408 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress