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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from common myeloid progenitor cells (CMP) of C57BL6 mouse

ABSTRACT: Common myeloid progenitor cells from murine bone marrow were sorted according to ROS content using FACS with H2-DCFDA staining. We compared gene expression profile between CMP with low-ROS levels and those with high-ROS levels. We analysed function of ROS in differentiation step from CMP to MEP.

ORGANISM(S): Mus musculus

SUBMITTER: Akihito Shinohara

PROVIDER: E-GEOD-57469 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Intracellular reactive oxygen species mark and influence the megakaryocyte-erythrocyte progenitor fate of common myeloid progenitors.

Shinohara Akihito A Imai Yoichi Y Nakagawa Masahiro M Takahashi Tsuyoshi T Ichikawa Motoshi M Kurokawa Mineo M

Stem cells (Dayton, Ohio) 20140201 2

While most studies regarding reactive oxygen species (ROS) focus on their deleterious biological effects, a growing body of evidence indicates the importance of ROS as critical mediators of several signaling pathways, including those involved in hematopoiesis. In this study, we show the critical role of ROS in lineage decision of myeloid progenitors. In megakaryocyte-erythrocyte progenitor cells (MEP), intracellular ROS levels were found to be as low as those in hematopoietic stem cells (HSC). I ...[more]

PMID: 24167091

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