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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcript Profiling of Common Bean Embryo Regions at Globular Stage Using Laser Capture Microdissection Coupled with RNA-Seq

ABSTRACT: We used laser capture microdissection (LCM) to capture globular stage common bean embryo proper and suspensor, and profiled the transcriptome of these two embryo regions using next-generation sequencing. Our long-term goal is to understand the region-specific differentiation processes that occur during early embryo development and how genes are activated specifically in the suspensor and embryo proper. Illumina sequencing of transcripts from laser-captured embryo proper and suspensor regions of common bean globular stage embryo. Two biological replicates were collected for each embryo region.

ORGANISM(S): Phaseolus vulgaris

SUBMITTER: Bob Goldberg

PROVIDER: E-GEOD-57535 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:We used laser capture microdissection (LCM) to capture globular scarlet runner bean embryo propers and suspensors, and profiled the transcriptomes of these two embryo regions using next-generation sequencing. Our long-term goal is to understand the region-specific differentiation processes that occur during early embryo development and how genes are activated specifically in the suspensor and embryo proper. Illumina sequencing of transcripts from laser-captured embryo proper and suspensor regions of scarlet runner bean globular stage embryos. Two biological replicates were collected for each embryo region.

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Transcript Profiling of Common Bean Embryo Regions at Globular Stage Using Laser Capture Microdissection Coupled with RNA-Seq

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Transcript Profiling of Soybean Seed Compartments Using Laser Capture Microdissection Coupled With RNA-Seq

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Genome-wide identification of the Phaseolus vulgaris sRNAome using small RNA and degradome sequencing [smallRNA-Seq].

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