MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation.

ABSTRACT: MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by Ago2 CrossLinking ImmunoPrecipitation coupled with sequencing (CLIP-seq), fu

ORGANISM(S): Mus musculus

SUBMITTER: Bo Yang 

PROVIDER: E-GEOD-57596 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress