Target mRNAs induce tailing and trimming of Ago2-loaded miRNAs in neurons

ABSTRACT: We show that target mRNAs trigger the non-templated addition of nucleotides (mainly adenosines and uridines) and shortening of their cognate miRNAs in primary hippocampal neurons. The induced effect is observable both in total RNA and in Ago2-associated RNA, demonstrating that the process is initiated in Ago2 bound miRNAs. Illumina Small RNA sequencing was performed on rat hippocampal neurons infected with different target mRNAs in different conditions. The data includes 3 independent experiments: 1. Hippocampal neurons (DIV15) 6 days after transducing them with target mRNAs (driven by the Synapsin promoter) against different miRNAs. Samples are titled "Syn-Target". Samples transduced with targets containing extensive complementarity are labeled "Ext" and those with See

ORGANISM(S): Rattus norvegicus

SUBMITTER: Dimos Gaidatzis 

PROVIDER: E-GEOD-57663 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress