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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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SIRT6 and SIRT1 contribute to circadian gene expression

ABSTRACT: Comparison of the hepatic circadian transcriptomes reveals that SIRT6 and SIRT1 separately control transcriptional specificity, and therefore, define distinctly partitioned classes of circadian genes. Livers from WT and SIRT6 KO mice, and livers from WT and SIRT1 KO mice, were harvested over the circadian cycle at ZT 0, 4, 8, 12, 16 and 20 for gene expression analysis.

ORGANISM(S): Mus musculus

SUBMITTER: Paolo Sassone-Corsi

PROVIDER: E-GEOD-57830 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Partitioning circadian transcription by SIRT6 leads to segregated control of cellular metabolism.

Masri Selma S Rigor Paul P Cervantes Marlene M Ceglia Nicholas N Sebastian Carlos C Xiao Cuiying C Roqueta-Rivera Manuel M Deng Chuxia C Osborne Timothy F TF Mostoslavsky Raul R Baldi Pierre P Sassone-Corsi Paolo P

Cell 20140701 3

Circadian rhythms are intimately linked to cellular metabolism. Specifically, the NAD(+)-dependent deacetylase SIRT1, the founding member of the sirtuin family, contributes to clock function. Whereas SIRT1 exhibits diversity in deacetylation targets and subcellular localization, SIRT6 is the only constitutively chromatin-associated sirtuin and is prominently present at transcriptionally active genomic loci. Comparison of the hepatic circadian transcriptomes reveals that SIRT6 and SIRT1 separatel ...[more]

PMID: 25083875

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Lung adenocarcinoma distally rewires hepatic circadian homeostasis

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2016-04-01 | E-GEOD-73222 | biostudies-arrayexpress

SIRT6 and SIRT7 binding proteome

Project description:To find the SIRT6 and SIRT7 binding proteins, we expressed V5-tagged SIRT6 and SIRT7 and pulled down this protein with anti-V5 agarose affinity gel. Sirtuins are a family of NAD-dependent deacetylases which deacetylate not only histones but also a wide range of target proteins. Sirtuins are conserved from yeast to mammal, and are known to regulate many processes such as cellular metabolism, apoptosis, cellular senescence, cell cycle, and organism-level aging. Among the members of sirtuins showing different subcellular localization, SIRT1, SIRT6 and SIRT7 are localized in nucleus. Whereas many functions and interacting proteins of SIRT1 have been studied, functions of SIRT6 and SIRT7 are not extensively revealed yet. Since it is important to understand the molecular function of SIRT6 and SIRT7, we here aimed to identify interacting proteins in mammalian cells.<br>Extra primary submitter: <a href="mailto:nglee@postech.ac.kr" target="_top">Namgyu Lee</a>, Department of Life Sciences, Pohang University of Science and Technology, Pohang, 790-784, Republic of Korea<br>Lab head: <a href="mailto:kchoi@postech.ac.kr" target="_top">Kwan Yong Choi</a>, Department of Life Sciences and Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, Pohang, 790-784, Republ ic of Korea

2014-04-28 | PXD000159 | Pride

SIRT6 suppresses pancreatic cancer through control of Lin28b [ChIP-seq]

Project description:Chromatin remodeling proteins are frequently dysregulated in human cancer, yet little is known about how they control tumorigenesis. Here, we uncover an epigenetic program mediated by the NAD+-dependent histone deacetylase Sirtuin 6 (SIRT6) that is critical for suppression of pancreatic ductal adenocarcinoma (PDAC), one of the most lethal malignancies. SIRT6 inactivation accelerates PDAC progression and metastasis via upregulation of Lin28b, a negative regulator of the let-7 microRNA. SIRT6 loss results in histone hyperacetylation at the Lin28b promoter, Myc recruitment, and pronounced induction of Lin28b and downstream let-7 target genes, HMGA2, IGF2BP1 and IGF2BP3. This epigenetic program defines a distinct subset representing 30-40% of human PDAC, characterized by poor prognosis and an exquisite dependence on Lin28b for tumor growth. Thus, we identify SIRT6 as an important PDAC tumor suppressor, and uncover the Lin28b pathway as a potential therapeutic target in a molecularlydefined PDAC subset. ChIP-Seq experiments to examine H3K56ac histone modifications in murine PDAC cells that are Sirt6 wild type (WT), Sirt6 knock-out (KO), and Sirt6 KO cells engineered to express Sirt6 WT (Sirt6 KO + Sirt6 WT Restored).

2016-04-01 | E-GEOD-79503 | biostudies-arrayexpress

SIRT6 and SIRT1 contribute to circadian gene expression

Project description:Comparison of the hepatic circadian transcriptomes reveals that SIRT6 and SIRT1 separately control transcriptional specificity, and therefore, define distinctly partitioned classes of circadian genes.

2014-08-05 | GSE57830 | GEO

Circadian Liver Gene Expression in Animals on Normal Chow or High Fat Diet

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2013-12-19 | E-GEOD-52333 | biostudies-arrayexpress

SIRT6 regulates redox homeostasis in human mesenchymal stem cells by the transactivation of NRF2

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2016-01-04 | E-GEOD-64642 | biostudies-arrayexpress

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2012-04-01 | E-MTAB-871 | biostudies-arrayexpress

SIRT6 regulates glucose metabolism and glutamatergic synapse in the mouse retina

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2014-07-30 | E-GEOD-56563 | biostudies-arrayexpress

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2011-07-30 | E-GEOD-28548 | biostudies-arrayexpress

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2012-08-01 | E-GEOD-38625 | biostudies-arrayexpress

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