Transcriptional patterns associated with OvCar-3 cells in response to osteopontin-c splicing isoform overexpression

ABSTRACT: We previously demonstrated that the osteopontin-c (OPNc) splice variant activates several aspects of the progression of ovarian cancers. The goal of the present study was to develop cell line model to determine the impact of OPNc overexpression on main cancer signaling pathways and thus obtain insights into the mechanisms of OPNc pro-tumorigenic roles. Methods: OvCar-3 cells were stably transfected to overexpress OPNc. Transcriptomic profiling was performed on these cells and compared to controls, to identify OPNc overexpression-dependent changes in gene expression levels and pathways by qRT-PCR analyses; Results: Of 84 genes tested by using a multiplex real-time PCR Cancer Pathway Array approach, 34, were differentially expressed between OvCar-3 OPNc-overexpressing cells in relation to

ORGANISM(S): Homo sapiens

SUBMITTER: Tatiana Tilli 

PROVIDER: E-GEOD-57888 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress