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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Streptomyces hygroscopicus 5008 Transcriptome or Gene expression

ABSTRACT: The RNA sequencing approach was utilized to carry out a comparative transcriptome analysis between Streptomyces hygroscopicus 5008 wild-type and a genetically engineered strain. The A-factor-like cascade play an important role in the regulation of validamycin biosynthesis by Streptomyces hygroscopicus 5008, and the pleiotropic regulator AdpA-H may positively regulate the transcription of gene cluster for the biosynthesis. shbR1 and shbR3 as the A-factor receptor homolog genes, could repress the transcription of AdpA-H. By tandem deletions of these genes, the production and productivity of validamcyin was significantly enhanced. To explore the effects of the shbR1/R3 double deletion of the overall cellular metabolism, the RNA sequencing approach was utilized to carry out a comparative transcriptome analysis between wild-type and shbR1/shbR3 double mutant (genetically engineered strain). The trancriptome analysis between 5008 and DM98 was carried out respectively.

ORGANISM(S): Streptomyces hygroscopicus subsp. jinggangensis

SUBMITTER: Gao-Yi Tan

PROVIDER: E-GEOD-58115 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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