Global characterization of the oocyte-to-embryo transition in C. elegans uncovers a novel mRNA turnover mechanism

ABSTRACT: The oocyte-to-embryo transition (OET) is thought to be mainly driven by post-transcriptional gene regulation. However, expression of both RNAs and proteins during the OET has not been comprehensively assayed. Furthermore, specific molecular mechanisms that regulate gene expression during OET are largely unknown. Here, we quantify and analyze, transcriptome-wide, expression of mRNAs, small RNAs and thousands of proteins in C. elegans oocytes, 1-cell, and 2-cell embryos. This represents a first comprehensive gene expression atlas during the OET in animals. We discovered a first wave of degradation in which thousands of mRNAs are turned over shortly after fertilization. Sequence analysis revealed a statistically highly significant presence of a novel polyC motif in the 3' untranslated regions

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Dominic Gruen 

PROVIDER: E-GEOD-58141 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress