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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from liver of Retnla transgenic (Tg) and non-transgenic (non-Tg) mice.

ABSTRACT: We found that Retnla-Tg mice had significantly lower serum cholesterol levels than non-Tg mice on a high-fat diet (HFD). To explore the molecular mechanisms underlying the cholesterol-lowering effects of Retnla under hyperlipidemic conditions, we subjected age- and sex-matched Retnla-Tg and non-Tg mice to a HFD for 4 weeks. Using a microarray approach, we analyzed the hepatic gene expression profiles related to cholesterol metabolism, including catabolism, biosynthesis, and transport. Total RNA was extracted from livers of Retnla-Tg and non-Tg mice after feeding with a HFD for four weeks, and transcriptional profiling was performed on individual samples using Affymetrix Mouse Genome chips (MG 430 2.0 array).

ORGANISM(S): Mus musculus

SUBMITTER: GooTaeg OH

PROVIDER: E-GEOD-58214 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression data from liver of Retnla transgenic (Tg) and non-transgenic (non-Tg) mice.

Project description:We found that Retnla-Tg mice had significantly lower serum cholesterol levels than non-Tg mice on a high-fat diet (HFD). To explore the molecular mechanisms underlying the cholesterol-lowering effects of Retnla under hyperlipidemic conditions, we subjected age- and sex-matched Retnla-Tg and non-Tg mice to a HFD for 4 weeks. Using a microarray approach, we analyzed the hepatic gene expression profiles related to cholesterol metabolism, including catabolism, biosynthesis, and transport.

2014-06-06 | GSE58214 | GEO

Adenosine-rich extract of Ganoderma lucidum: a safe and effective lipid-lowering substance via regulating expression and modulation of PPAR signaling pathway

Project description:Ganoderma lucidum is a traditional Chinese medicine with a variety of active compounds and possessing adequate lipid-lowering and anti-atherosclerotic effects. However, its main active components and potential mechanisms still remain unclear. Here, we evaluated the anti-hyperlipidemic effect of the adenosine extract from Ganoderma lucidum (AEGL) in high-fat-diet (HFD)-induced hyperlipidemic ApoE-/- mice and explored the underlying biological mechanism by multi-omics analysis. Treatment with AEGL for 8 weeks significantly decreased the serum levels of total cholesterol (TC), triglyceride (TG), and low-density lipoprotein cholesterol (LDL-c) by 45.59%, 41.22%, and 39.02%, respectively, as well as reduced liver TC and TG by 44.15% and 76.23%, compared with the HFD-only group. We also observed significant amelioration of hepatic steatosis without liver and kidney damage after AEGL treatment. Regulating the expression and acetylation/crotonylation of proteins involved in the PPAR signaling pathway may be one of the potential mechanisms involved in the observed lipid-lowering effects of AEGL.

2023-03-11 | PXD036478 | Pride

Proteomic detection of mouse liver

Project description:This study aimed to investigate the mechanism of SYT on lipid metabolism and insulin sensitivity in high-fat diet (HFD)-induced obese mice by means of combining lipidomics and proteomics. The obese mice models were developed via HFD feeding for 20 consecutive weeks. Mice in the treatment group were given metformin and SYT respectively, and the effects of SYT on body weight, blood glucose, insulin sensitivity, fat accumulation in the organs, and pathological changes in the liver were monitored. Lipid metabolism was examined by lipidomics. Further determination of signaling pathways was detected by proteomics. The biological contributions of the compounds detected in SYT's chemical fingerprint were predicted by network pharmacology. Overall, our study provides supportive evidence for the mechanism of SYT's therapeutic effect on dysregulated lipid metabolism in diabesity.

2022-02-15 | PXD028317 | Pride

Genome-wide expression profile of livers from mice fed HFD,SO-HFD or Viv chow [RNAseq]

Project description:Purpose: Aim of the study is to identify changes in hepatic gene expression induced by either a 40kcal% coconut oil rich high fat diet (HFD), a 40kcal% soybean oil plus coconut oil high fat diet (SO-HFD) or a low fat vivarium chow diet (Viv). Methods: Livers from mice that had been fed one of the above mentioned diets for 35 weeks, were used to make cDNA libraries that were then sent for deep sequencing, using the Illumina TruSeq RNA. Result: Many genes involved in metabolism, lipid binding, transport and storage and many Cyp genes are dysregulated in the two high fat diets as compared to Viv HFDs in SO-HFD mice. Comparing the two HFDs shows more metabolism and disease related genes dysregulated in SO-HFD vs HFD. Conclusion: A diet high in soybean oil may be more detrimental to metabolic health than a diet high in saturated fats. cDNA isolated from livers from mice fed HFD, SO-HFD or Viv for 35 weeks, were 50bp pair-ended sequenced in triplicate using Illumina TruSeq RNA Sample Prep v2 Kit.

2015-08-28 | E-GEOD-68360 | biostudies-arrayexpress

Microarray analysis of transgenic mice specifically overexpressing FOXO1 in skeletal muscle (FOXO1 Tg mice)

Project description:FOXO1, a member of the FOXO forkhead type transcription factors, is markedly up-regulated in skeletal muscle during atrophy. Previously, we created transgenic mice specifically overexpressing FOXO1 in skeletal muscle (FOXO1 Tg mice). These mice weighed less than the wildtype control mice, had a reduced skeletal muscle mass. In this study, to better understand changes in skeletal muscle during atrophy, we performed a microarray analysis of skeletal muscle in wild-type control and FOXO1 Tg mice. The microarray data shows that in the skeletal muscles of FOXO1 Tg mice, gene expression of PGC-1β, a transcriptional regulator whose increased expression activates energy-expenditure-related genes in skeletal muscles, is decreased.

2020-07-07 | GSE146919 | GEO

Effect of maple syrup extract on the liver of mice fed a high-fat diet

Project description:The effects of the administration of maple syrup extract (MSX) on hepatic gene expression were investigated in mice fed high-fat diet. Male C57BL/6J mice aged 3 weeks were purchased from Charles River Japan (Kanagawa, Japan) and housed in a room maintained at 23 ± 1°C and 49 ± 16% humidity with a 12-h light/dark cycle (light 08:00–20:00; dark 20:00–08:00). For 1 week acclimation period after purchase, all the mice were fed a low-fat diet (10 kcal% fat). Then, they were randomly divided into three different dietary groups: the first group with the food containing fat at 10 kcal% as low-fat diet, the second group with 45 kcal% as high-fat diet, and the third with HFD plus 0.06% MSX. The mice were fed ad libitum for 8 weeks.

2015-04-30 | E-GEOD-65667 | biostudies-arrayexpress

Transcription profiling of mouse liver isolated from high-fat diet feeded WT and LIGHT Tg mice

Project description:effect of over-expression LIGHT on T cells for the liver gene expression; We used microarrays to detail the global programme of gene expression underlying WT and LIGHT Tg liver Experiment Overall Design: mice were selected at 4-8 wks old after 2 weeks HFD feeding for RNA extraction and hybridization on Affymetrix microarrays. The total liver were used.

2008-06-15 | E-GEOD-6903 | biostudies-arrayexpress

Expression data for Tcl1 tg mice compared to CD44ΔB Tcl1 tg mice

Project description:Tcl1 tg mice develop a chronic lymphocytic leukemia (CLL) -like disease. To investigate the contribution of the adhesion molecule CD44 to CLL pathophysiology, we developed a CD19Cre CD44flox/flox Tcl1 tg mouse with a B cell specific CD44 deficiency (CD44ΔB Tcl1 tg). We used the Clariom S mouse microarray from Affymetrix to investigate transcriptional differeneces between Tcl1 tg and CD44ΔB Tcl1 tg mice

2018-01-13 | GSE109121 | GEO

Expression data for Tcl1 tg mice compared to CD44ΔB Tcl1 tg mice

Project description:Expression data for Tcl1 tg mice compared to CD44ΔB Tcl1 tg mice

| PRJNA429732 | ENA

Gene expression profile of splenic CD19 cells from NTg, PTPROt Tg, TCL1 Tg and PTPROt/TCL1 double Tg mice

Project description:TCL1 is an an oncogene and transgenic (Tg) mice expressing TCL1 specifically in B-cells are well-characterized models for chronic lymphocytic leukemia. On the contrary, PTPROt is a phosphatase with tumor suppressor characteristics in many cancers including leukemia. Our hypothesis was that transgenic expression of PTPROt in the B-cells of TCL1 Tg mice will alleviate disease phenotype and allow the study of the in vivo mechanism of action of PTPROt. To test this we have generated Tg mice with B-cell specific expression of PTPROt and crossed these mice with the TCL1 Tg mice.

2016-01-04 | GSE60582 | GEO

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