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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Multi-Omic Integrated networks connect DNA methylation and miRNA with skeletal muscle plasticity to chronic exercise in type 2 diabetic obesity [mRNA data]

ABSTRACT: Analysis of skeletal muscle gene expression from type 2 diabetic volunteers before and after 16 weeks of chronic exercise training (two groups, one undergoing aerobic ecercise and the other resistance training exercise) A biopsy was collected from the right Vastus Lateralis under local anaesthesia and RNA was isolated from muscle (~10 mg) using mirVana™ Kit , 200 ng RNA from each sample was used for mRNA expression profiling using HumanHT-12_v4_BeadChip (Illumina). Arrays were scanned using HiScanSQ System. Decoded images were analyzed by GenomeStudio™ (Illumina).

ORGANISM(S): Homo sapiens

SUBMITTER: David Rowlands

PROVIDER: E-GEOD-58249 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Multi-Omic Integrated networks connect DNA methylation and miRNA with skeletal muscle plasticity to chronic exercise in type 2 diabetic obesity [Methylation data]

Project description:Analysis of skeletal muscle DNA methylation from type 2 diabetic volunteers before and after 16 weeks of chronic exercise training (two groups, one undergoing aerobic excercise and the other resistance training exercise) A biopsy was collected from the right Vastus Lateralis under local anaesthesia andGenomic DNA was extracted from 5-10 mg muscle, Bisulphite conversion (Illumina) was checked using methylation specific PCR. 4 ?l of bisulphite-converted DNA was used for hybridization on Infinium Human Methylation 450 BeadChip (Illumina)

2014-09-10 | E-GEOD-58280 | biostudies-arrayexpress

Multi-Omic Integrated networks connect DNA methylation and miRNA with skeletal muscle plasticity to chronic exercise in type 2 diabetic obesity [miRNA data]

Project description:Analysis of skeletal muscle miRNA expression from type 2 diabetic volunteers before and after 16 weeks of chronic exercise training (two groups, one undergoing aerobic ecercise and the other resistance training exercise) A biopsy was collected from the right Vastus Lateralis under local anaesthesia and RNA was isolated from muscle (~10 mg) using mirVanaM-bM-^DM-" Kit , Low molecular weight RNA was biotin-labeled with FlashTagM-bM-^DM-" Biotin HSR RNA Labeling Kit. 21.5 M-BM-5l of high-quality biotin-labeled miRNA sample was hybridized to Affymetrix Gene-ChipM-BM-. miRNA 2.0, stained on Affymetrix Fluidics station 400 and scanned with a Hewlett Packard G2500A gene Array Scanner. AffymetrixM-BM-. miRNA QC Tool v 1.1.1.0 was used for the data summarization, normalization, and microarray quality control.

2014-09-10 | E-GEOD-58248 | biostudies-arrayexpress

Multi-Omic Integrated networks connect DNA methylation and miRNA with skeletal muscle plasticity to chronic exercise in type 2 diabetic obesity [Methylation data]

2014-09-10 | GSE58280 | GEO

Multi-Omic Integrated networks connect DNA methylation and miRNA with skeletal muscle plasticity to chronic exercise in type 2 diabetic obesity [mRNA data]

Project description:Analysis of skeletal muscle gene expression from type 2 diabetic volunteers before and after 16 weeks of chronic exercise training (two groups, one undergoing aerobic ecercise and the other resistance training exercise)

2014-09-10 | GSE58249 | GEO

Multi-Omic Integrated networks connect DNA methylation and miRNA with skeletal muscle plasticity to chronic exercise in type 2 diabetic obesity [miRNA data]

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