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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression profiling in MCF-7 cells exposed to blackcurrant extract.

ABSTRACT: Blackcurrants (Ribes nigrum L., Grossulariaceae) have a high content of anthocyanin polyphenols and these have been shown to have beneficial effects on health, owing to their antioxidant and anti-carcinogenic properties. This study analyzed the constituents of blackcurrant extract (BCE) and investigated its potential phytoestrogenic effects using a breast cancer cell line (MCF-7) overexpressing the estrogen receptor (ER) Î±. Microarray and ingenuity pathway analysis showed that BCE activated upstream genes such as ERÎ± and transforming growth factor beta 1, and upregulated the expression of many genes downstream of ERÎ±. MCF-7 cells were seeded in culture dish and maintain to confluent. Then medium replace with phenol-red-serum-free DMEM medium with or without BCE (50Î¼g/ml). After the cells were incubated for 24 h at 37 Â°C 5% CO2.

ORGANISM(S): Homo sapiens

SUBMITTER: Naoki Nanashima

PROVIDER: E-GEOD-58304 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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