Foxd3 binding at enhancers controls developmental timing of gene expression by regulation of histone acetylation [expression array]

ABSTRACT: Transcription factor/enhancer interactions determine cell specific gene expression. Here, we followed enhancers during differentiations of embryonic stem (ESCs) to epiblast like cells (EpiLCs). There were highly dynamic changes in histone lysine 27 acetylation at enhancer sites throughout the genome. These sites were enriched for a Foxd3 binding motif, a forkhead transcription factor essential in early embryonic development. Surprisingly, Foxd3 occupied largely mutually exclusive sites in the ESCs versus EpiLCs. Foxd3 bound to nucleosome occupied regions, simultaneously evicting the histones while inhibiting full gene expression through the recruitment of histone deacetylases. Knockout of Foxd3 resulted in hyperacetylation and transcriptional upregulation of neighboring genes, many of wh

ORGANISM(S): Mus musculus

SUBMITTER: Raga Krishnakumar 

PROVIDER: E-GEOD-58317 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress