Next Generation Sequencing and Quantitative Analysis of Wild Type and p110gamma-/- macrophages

ABSTRACT: Purpose: The goals of this study were to identify quantitative gene expression differences between macrophages derived from wild type and PI3Kgamma null macrophages Methods: mRNA profiles of MCSF, IL4 and IFNg/LPS stimulated macrophage wild-type (WT) and PI3Kinase gamma knockout (p110g-/-) mice were generated by single read deep sequencing, in triplicate, using Illumina HiSeq2000. The sequence reads that passed quality filters were aligned to mouse transcriptome using the bowtie2 aligner. Gene-level summaries were normalized and analyzed for differential expression using DESeq. qRT–PCR validation was performed using SYBR Green assays. Conclusions: Our study represents the first detailed analysis of the role of p110g in the control of the macrophage immune response, with biological repl

ORGANISM(S): Mus musculus

SUBMITTER: Roman Sasik 

PROVIDER: E-GEOD-58318 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress