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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from mouse spleen CD11c dendritic cells, bone marrow Gr1DC11b cells, and RAW264.7 cells

ABSTRACT: We investigated in mouse models how enhanced coagulation activation due to a common disease polymorphism in coagulation factor V (fV Leiden Arg506Gln) modifies the host response to infection and inflammation We used microarrays to characterize the in vivo and in vitro inflammatory responses of mouse spleen dendritic cells, macrophage-like RAW264.7 cells, and bone marrow myeloid cells Samples 1-6 were generated by pooling equal amounts of RNA prepared from FACS-enriched spleen dendritic cells isolated from 6 individual mice before (control sample 1) or 16 hours after challenge with lipopolysaccharide. Samples 7-10 were generated by pooling equal amounts of RNA prepared from triplicate cultures of RAW264.7 cells. Samples 10-14 (2 replicas per condition) were prepared from RNA isolated from FACS-enriched bone marrow myeloid cells (pooled from 4 adoptively transferred animals each) 7 days after infection with S.aureus.

ORGANISM(S): Mus musculus

SUBMITTER: Hartmut Weiler

PROVIDER: E-GEOD-58328 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression data from mouse spleen CD11c dendritic cells, bone marrow Gr1DC11b cells, and RAW264.7 cells

Project description:We investigated in mouse models how enhanced coagulation activation due to a common disease polymorphism in coagulation factor V (fV Leiden Arg506Gln) modifies the host response to infection and inflammation We used microarrays to characterize the in vivo and in vitro inflammatory responses of mouse spleen dendritic cells, macrophage-like RAW264.7 cells, and bone marrow myeloid cells

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