Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Breast cancer tumor promoting cell transcriptome

ABSTRACT: In this study we obtained gene expression profiles of MCFS and parental MCF7 cell lines using Illumina microarrays Transcriptome analysis of tumor promoting mammospheres (MCFS) and parental MCF7 breast cancer cell line was run in triplicate on microarrays

ORGANISM(S): Homo sapiens

SUBMITTER: Maurizio Callari

PROVIDER: E-GEOD-58383 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

In-depth characterization of breast cancer tumor-promoting cell transcriptome by RNA sequencing and microarrays.

Callari Maurizio M Guffanti Alessandro A Soldà Giulia G Merlino Giuseppe G Fina Emanuela E Brini Elena E Moles Anna A Cappelletti Vera V Daidone Maria Grazia MG

Oncotarget 20160101 1

Numerous studies have reported the existence of tumor-promoting cells (TPC) with self-renewal potential and a relevant role in drug resistance. However, pathways and modifications involved in the maintenance of such tumor subpopulations are still only partially understood. Sequencing-based approaches offer the opportunity for a detailed study of TPC including their transcriptome modulation. Using microarrays and RNA sequencing approaches, we compared the transcriptional profiles of parental MCF7 ...[more]

PMID: 26556871

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Project description:Human toxicogenomic studies to date have been of limited size, have mainly addressed exposures at the upper end of typical ranges of human exposure, and have often lacked precise, individual estimates of exposure. Previously, we identified genes associated with exposure to high (>10 ppm) levels of the leukemogen, benzene, through transcriptomic analyses of blood cells from small numbers of occupationally exposed workers. Here, we have expanded the study to 125 workers exposed to a wide range of benzene levels, including <1 ppm. Study design, and analysis with a mixed effects model, removed sources of biological and experimental variability and revealed highly significant widespread perturbation of gene expression at all exposure levels. Benzene is an established cause of acute myeloid leukemia (AML), and may cause one or more lymphoid malignancies in humans. Interestingly, acute myeloid leukemia was among the most significant pathways impacted by benzene exposure in the present study. Further, at most exposure levels, immune response pathways including T cell receptor signaling, B cell receptor signaling, and Toll like receptor signaling were impacted, providing support for the biological plausibility of an association between lymphoma and benzene exposure. We also identified a 16-gene expression signature modified by all levels of benzene exposure, comprising genes with roles in immune response, inflammatory response, cell adhesion, cell-matrix adhesion, and blood coagulation. Overall, these findings support, and expand upon, our current understanding of the mechanisms by which benzene may induce hematotoxicity, leukemia and lymphoma. Furthermore, this study shows that with good study design and analysis, transcriptome profiling of the blood of chemically-exposed humans can identify relevant biomarkers across a range of exposures and inform about potential associations with disease risks. Key words: occupational exposure 29 workers exposed to <<1ppm benzene at most dosimetry reading over a 14-month period (Very Low), 30 exposed to average <1ppm (Low), 11 exposed to 5-10 ppm (High), 13 exposed to >10 ppm (Very High), and 42 unexposed controls (Control) who were frequency-matched to these subjects on the basis of age and gender were analyzed (technical replicates on some subjects, see SubjectID/description).

Dataset Information

Breast cancer tumor promoting cell transcriptome

Publications

In-depth characterization of breast cancer tumor-promoting cell transcriptome by RNA sequencing and microarrays.

Similar Datasets

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