Differential gene expression in the skin of dogs sensitized to the house dust mite Dermatophagoides farinae

ABSTRACT: Atopic dermatitis is a multifactorial allergic skin disease in humans and dogs. Genetic predisposition, immunologic hyperreactivity, a defective skin barrier and environmental factors play a role in its pathogenesis. The aim of this study was to analyze gene expression in the skin of dogs sensitized to house dust mite antigens. Skin biopsies were collected from six sensitized and six non-sensitized Beagle dogs from normal, non-treated skin before and six and 24 hours after challenge using skin patches with allergen or saline as a negative control. Transcriptome analysis was performed by the use of DNA microarrays and expression of selected genes was validated by quantitative real-time RT-PCR. Expression data was compared between groups (unpaired design). After 24 hours 597 differentially e

ORGANISM(S): Canis lupus familiaris

SUBMITTER: Stefan Bauersachs 

PROVIDER: E-GEOD-58442 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress