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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Coilin's RNA, DNA and protein interactomes redefine the Cajal body as a hub for small non-coding RNAs

ABSTRACT: This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

ORGANISM(S): Homo sapiens

SUBMITTER: Karla Neugebauer

PROVIDER: E-GEOD-58451 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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iCLIP mapping of coilin-RNA interactions in HeLa and P19 cells

Project description:Our ChIP resuls suggested that coilin association with U3, snRNA and histone genes might be dependent on coilin-RNA interaction. We used iCLIP of coilin-GFP expressed in HeLa and P19 cell lines at endogenous levels to identify coilin RNA targets and investigate RNA-binding specificity. P19 cells expressing GFP fused to a nuclear localization signal (GFP-NLS) was used as a negative control. iCLIP results revealed that coilin binds several classes of ncRNA including snRNAs, U3 snoRNA and scaRNAs. Interestlignly the majority of coilin targets were intronic snoRNAs, suggesting a novel role of CBs in snoRNA biogenesis. 5 biological replicates from P19 and 2 biological replicates from HeLa cells after UV-crosslinking. Negative control samples prepared from GFP-NLS fusion protein are stored uder accession E-MTAB-747.

2014-11-06 | E-GEOD-58448 | biostudies-arrayexpress

Coilin ChIP-seq in cycling HeLa cells

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Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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